Seven Hills Bioreagents

Ad5-CMV-p38beta-GFP fusion protein

Download DatasheetDownload datasheet
Catalog NumberJMAd-46
Quantity0.5 ml
Category
  • MAP Kinase Related

Genep38-beta-eGFP fusion protein (cell localization studies)
Vector ConstructionHuman p38beta cDNA fused to enhanced GFP was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human p38beta cDNA fused to enhanced GFP expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingImmunofluorescence tested
TiterApprox 1E8/ml
References
Unpublished.

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

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