Genep38-beta-mutant estrogen receptor fusion (fusion protein activated by tamoxifen) Vector ConstructionHuman p38-beta cDNA fused to mutant estrogen receptor was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. This generates a tamoxifen inducible version of p38 beta. Supplied AsCrude HEK293 cell lysate. StorageStore at -80°C. Multiple freeze/thaw cycles not recommended. Epitope TagNone QC TestingWestern Blot TiterApprox 1E8/ml
References Unpublished.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
Dong M et al.
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Mol. Biol. Cell 2008;19:2620-2630.
Stahlman MT et al.
Expression of ABCA3 in developing lung and other tissues. J Histochem Cytochem 2007;55:71-83.