Ad5-CMV-MEF2C-DN-full R3T
Download datasheetCatalog NumberJMAd-31
Quantity0.5 ml
Category
- Transcription Factors
GeneMEF2C-DN-full R3T
Vector ConstructionThe dominant negative mutant of MEF2C (myocyte enhancer factor 2) mouse full-length with R3T mutation was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse full-length mutation expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Xu J, Gong NL, Bodi I, Aronow BJ, Backx PH, Molkentin JD. MEF2A and MEF2C induce dilated cardiomyopathy in transgenic mice. J. Biol. Chem. 2006. In press.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
Xu J, Gong NL, Bodi I, Aronow BJ, Backx PH, Molkentin JD. MEF2A and MEF2C induce dilated cardiomyopathy in transgenic mice. J. Biol. Chem. 2006. In press.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
