Seven Hills Bioreagents

Ad5-CMV-p38alpha-mutant estrogen receptor fusion

Download DatasheetDownload datasheet
Catalog NumberJMAd-47
Quantity0.5 ml
Category
  • MAP Kinase Related

Genep38-alpha-mutant estrogen receptor fusion (fusion protein activated by tamoxifen)
Vector ConstructionHuman p38alpha cDNA fused to mutant estrogen receptor was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. This generates a tamoxifen inducible version of p38 beta.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Unpublished.

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

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