Ad5-CMV-TRPC4
Download datasheetCatalog NumberJMAd-113
Quantity0.5 ml
Category
- Miscellaneous
GeneTRPC6
Vector ConstructionMouse cDNA for TRPC4 cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cDNA TRPC4 expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Wu X, Eder P, Chang B, Molkentin JD.
TRPC channels are necessary mediators of pathologic cardiac hypertrophy. Proc Natl Acad Sci USA. 107:7000-7005.
2010 Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
Wu X, Eder P, Chang B, Molkentin JD.
TRPC channels are necessary mediators of pathologic cardiac hypertrophy. Proc Natl Acad Sci USA. 107:7000-7005.
2010 Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
