Seven Hills Bioreagents

Anti-Actin - Mouse (C4)

C4 reacts with all known actins from yeast to man with no known spurious reactivities. The C4 antibody is an excellent loading control for Western blots.

Download DatasheetDownload datasheet
Catalog NumberLMAB-C4
Quantity100 μl
Category
  • Cytoskeletal Antibodies
  • Monoclonal

SpecificityAll known actins from yeast to man
ImmunogenChicken gizzard actin
IsotypeIgG1/Kappa
Applications
& suggested dilutionsApplications &suggested dilutions Immunoblots - 1:500 (DAB/peroxidase detection)Indirect immunofluorescence - 1:500Immunohistochemistry - 1:500, Blocking conditions: human specimens: 2% serum, mouse specimens: MOM kit (Vector Labs)
Supplied AsAscites fluid containing 0.01% Na azide.
StorageStorage at -70°C recommended, however the antibody is stable for months at -20°C and for weeks at 4°C. Multiple freeze/thaw cycles not recommended.
References
Lessard, JL. 'Two monoclonal antibodies to actin: one muscle selective and one generally reactive.' Cell Motil Cytoskeleton. 1988;10(3):349-62.

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