Ad5-CMV-calcineurin inhibitory AKAP79 protein
Download datasheetCatalog NumberJMAd-06
Quantity0.5 ml
Category
- Calcineurin/NFAT
Gene calcineurin inhibitory AKAP79 peptide (amino acids 60-358)
Vector ConstructionHuman cDNA for AKAP79 amino acids 60-358 (calcineurin inhibitory domain) with 5’ flag epitope cDNA was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human cDNA for AKAP79 amino acids 60-358 (calcineurin inhibitory domain) with 5’ flag epitope expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagFlag
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Taigen T, De Windt LJ, Lim HW, Molkentin JD. Targeted inhibition of calcineurin prevents agonist-induced cardiomyocyte hypertrophy. Proc. Natl. Acad. Sci. USA. 97:1196-1201.2000.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
Taigen T, De Windt LJ, Lim HW, Molkentin JD. Targeted inhibition of calcineurin prevents agonist-induced cardiomyocyte hypertrophy. Proc. Natl. Acad. Sci. USA. 97:1196-1201.2000.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
