Seven Hills Bioreagents

Ad5-CMV-JNK1-mutant estrogen receptor fusion

Download DatasheetDownload datasheet
Catalog NumberJMAd-53
Quantity0.5 ml
Category
  • MAP Kinase Related

GeneJNK1-mutant estrogen receptor fusion (tamoxifen-inducible)
Vector ConstructionMouse C-Jun N-terminal kinase (JNK1) cDNA fused to the mutant estrogen receptor was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. This generates a tamoxifen inducible version of JNK1.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Unpublished.

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

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