Seven Hills Bioreagents

Ad5-CMV-p38beta wild type

Download DatasheetDownload datasheet
Catalog NumberJMAd-41
Quantity0.5 ml
Category
  • MAP Kinase Related

Genep38beta wildtype
Vector ConstructionHuman cDNA for wildtype p38beta with 5’ flag epitope was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human cDNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagFlag
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Unpublished

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

Products

Newsletter

Sign up to receive our newsletter, updates and our latest product releases.

We will not share, rent, or give your name or email address to anyone else. Please see our privacy policy if you have any questions.

Recently Published Articles

Chen G, Korfhagen TR, Karp CL, Impey S, Xu Y, Randell SH, Kitzmiller J, Maeda Y, Haitchi HM, Sridharan A, Senft AP, Whitsett JA.
Foxa3 induces goblet cell metaplasia and inhibits innate antiviral immunity. Am J Respir Crit Care Med. 2014 Feb 1;189(3):301-13.

Balli D, Ustiyan V, Zhang Y, Wang IC, Masino AJ, Ren X, Whitsett JA, Kalinichenko VV, Kalin TV.
Foxm1 transcription factor is required for lung fibrosis and epithelial-to-mesenchymal transition. EMBO J. 2013 Jan 23;32(2):231-44.

Dong M et al.
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Mol. Biol. Cell 2008;19:2620-2630.

Stahlman MT et al.
Expression of ABCA3 in developing lung and other tissues. J Histochem Cytochem 2007;55:71-83.