Anti-Smooth Muscle Actin - Mouse (B4)
B4 reacts selectively with enteric smooth muscle gamma-actin at high dilutions and does not react to either cytoplasmic ß- or gamma-actin.
Download datasheetCatalog NumberLMAB-B4
Quantity100 μl
Category
- Cytoskeletal Antibodies
- Monoclonal
SpecificityReacts selectively with enteric smooth muscle
ImmunogenChicken gizzard actin
IsotypeIgG1/Kappa
Applications
& suggested dilutionsTo be selective for smooth muscle actin: Immunoblots - 1:2000
Indirect immunofluorescence - 1:100-1:500
Immunohistochemistry - 1:500
Blocking conditions: human specimens: 2% serum
mouse specimens: MOM kit (Vector Labs)
Supplied AsAscites fluid containing 0.01% Na azide.
StorageStore at -70°C. Multiple freeze/thaw cycles not recommended.
References
Lessard, JL. 'Two monoclonal antibodies to actin: one muscle selective and one generally reactive.' Cell Motil Cytoskeleton. 1988;10(3):349-62.
Sawtell NM, Lessard JL. 'Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.' J Cell Biol. 1989 Dec;109(6 Pt 1):2929-37.
Lessard, JL. 'Two monoclonal antibodies to actin: one muscle selective and one generally reactive.' Cell Motil Cytoskeleton. 1988;10(3):349-62.
Sawtell NM, Lessard JL. 'Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.' J Cell Biol. 1989 Dec;109(6 Pt 1):2929-37.
