Anti-TTF1 - Rabbit
TTF-1 is expressed in airway epithelial cells during lung morphogenesis. This is a newly available rabbit antiserum that works well in immunohistochemistry.
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Catalog NumberWRAB-1231
Quantity100 μl
Category
Specificityhuman & mouse TTF-1, other species not tested
Immunogen recombinant rat TTF-1 6his fusion
IsotypeN/A
Applications
& suggested dilutionsImmunoblots - 1:5000, Immunohistochemistry - 1:1000 to 1:2000, Antigen retrieval: required (see IHC protocol), Blocking condition: 6% block (see IHC protocol)
Supplied AsRabbit serum containing 0.01% sodium azide.
Storage Store at -20°C. Multiple freeze/thaw cycles not recommended.
Download datasheetCatalog NumberWRAB-1231
Quantity100 μl
Category
- Lung Associated Antibodies
- Polyclonal
Specificityhuman & mouse TTF-1, other species not tested
Immunogen recombinant rat TTF-1 6his fusion
IsotypeN/A
Applications
& suggested dilutionsImmunoblots - 1:5000, Immunohistochemistry - 1:1000 to 1:2000, Antigen retrieval: required (see IHC protocol), Blocking condition: 6% block (see IHC protocol)
Supplied AsRabbit serum containing 0.01% sodium azide.
Storage Store at -20°C. Multiple freeze/thaw cycles not recommended.
References
Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
